Lactobacillus and electrons
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There’s a lot of discussion about what color clay to use for seed balls. We discuss some of the theory behind that here, but thought it would be a good idea to put some of that to the test. I wondered if we could change red clay into gleyed clay and back again.
Here is the science question: Can the conversion of Ferric Iron (Fe+3) into Ferrous Iron (Fe+2) be observed in a reasonably short time frame, with the naked eye, and with reasonably common supplies? Is the reaction reversible in a similar timeframe?
Here’s what I did to investigate:
The color of iron in the clay will most quickly change from red to gleyed in the presence of larger microbial populations, a food source, and anaerobic conditions.
Gleyed clay will revert to reddish-orange when exposed to aerobic conditions.
I had some gray low-fire clay around from my sculpture days. When I made a sculpture, I often used iron wires to hold the clay in place. They left rusty stains on the clay. Here you can see two dried chunks of clay, one with iron stains and one without. The red stains are Fe2O3, or iron oxide (aka rust). The iron in rust is Ferric and has the charge of +3.
I then powdered the clay, some gray and some rusty, in a mortar and pestle.
The first treatment is the control. I set aside two containers of powdered clay, with no additives. This will serve as a color reference.
The second treatment is 10g clay and 15g water, placed in a sealed container. This will show how the color of the clay changes with water. It is not sterile clay, but will have fewer microbes and less food for them than the final treatment.
The final treatment is 10g clay, 15g water, and 5g bokashi starter in a sealed container. It will show how the color of the clay changes when in the presence of Lactobacillus and other anaerobic microbes, and a food (electron) source, the bran in the bokashi starter.
t=1 day (Bokashi bran treatment on the right- not a lot of change yet- just a bit of darkening)
t=2 days: Beginning to see a difference here
The difference in color of the iron rich samples is becoming very apparent. The sample that has less iron also has some color changes.
t=3 days through 4 days
Increasing changes progressively observed.
Significant color changes in the both the iron-rich and iron-poor inoculated samples. The black colors, especially in the iron-poor sample suggests sulfur reduction, a good indicator that the Iron has been fully reduced.
t=5 days – opened for drying
Here you can see the top suface of the samples upon opening. Whew! Did it stink! Definitely sulfur reduction and the rotten eggy release of H2S. Un-inoculated samples indicated little change through the 5 days.
t=5.5 days – opened for drying 0.5 days
I expected the colors to begin changing reflecting oxidation, but not so rapidly! You can see the top layer of the iron-rich inoculated specimen has already changed to its ferric state.
t=7 days – opened for drying 2 days
Two days after drying, the iron-poor sample with inoculant is showing signs of oxidation. The black color is disappearing as the sulfur is oxidizing, and the stink has dissipated. A skin has formed on that sample that seems to slow the drying. The iron-rich inoculated sample is nearly completely dry.
t=10 days – opened for drying 5 days
Five days after drying, the iron-poor sample with inoculant continues to oxidize. The iron-rich inoculated sample is completely oxidized and dry, bearing close resemblance to the sample that was not inoculated.
t=12 days – opened for drying 7 days
All samples are dry and fully oxidized. The inoculated specimens again look much like the specimens without inoculant and the controls without water, even the sample that formed the tough skin.
Both hypotheses have been verified. Redox in clay as indicated by color changes and smell is rapidly induced through the presence of anaerobic conditions, bacteria and a food source and reversed by the addition of air at the seed-ball scale. Just anaerobic conditions were not sufficient to bring about reduction.
Based on these conclusions, it is reasonable to suspect that when seed balls are made, regardless of the color of the clay, the addition of live compost and water coupled with slow drying might begin to induce some redox conditions. Any redox that has been induced will be reversed as the seed balls dry. Both red and gray clay are capable of undergoing reduction and oxidation. The claim that grey clay should not be used on the basis of oxidation state should be weighed with some skepticism.